#ice #bath #muscle #growth

You have probably seen footage of well-built athletes such as Usain Bolt, Anthony Joshua, or Cristiano Ronaldo that have immersed (parts of) their body into ice cold water. There has

been this interesting interview with Football Manager Carlo Ancelotti about Cristiano

Ronaldo during his time at Real Madrid F.C. “…Cristiano Ronaldo, a self-motivating near-

cyborg who took 3am ice baths in the Real Madrid’s training complex. “Even though he had

Irina Shayk waiting for him at home!” Ancelotti yelps, referring to the Portuguese’s former

lover…” (1).

Given that these top athletes use cold-water immersion (even during the night), it has been

one of the most popular and frequently applied recovery strategies. However, if your aim is

to increase gains in muscle mass, how good is it then to immerse yourself in such an ice

bath? Will it help or actually hinder the capacity for muscle growth? Before answering that question let’s first look (briefly) at the impact of cooling on our body.

Impact of cooling the body

It is well-documented that cooling the body after exercise reduces both muscle temperature

and blood flow. Given these physiological changes, it has been claimed that this will improve the post-exercise recovery process. Indeed, there is evidence that cooling after exercise can decrease sensations of muscle soreness, likely because cold-induced decrements in tissue temperature lower acetylcholine production and nerve conduction velocity, which exerts an analgesic (pain reducing) effect. Furthermore, post-exercise cooling has been shown to lower muscle swelling and improve exercise performance in a subsequent exercise session (note: in some, but not all, studies). So, yes there could be reasons for athletes to cool the body after exercise. However, there is also data to suggest that cooling may not be beneficial for post-exercise recovery and/or that the possible benefits are (for a large extent) due to a placebo effect (i.e., it feels good “and works” for someone merely because that person has a belief that it works) (see discussion section of (2) for more information and references).

“Cooling may not be beneficial for post-exercise recovery and … the possible benefits are (for a large extent) due to a placebo effect”

Although cooling may have benefits for some aspects of recovery, recent evidence has

shown that post-exercise cooling could in fact be detrimental when the aim is to build our

muscle mass. Previous work has shown that both anabolic signaling and ribosomal

biogenesis is impaired following post-exercise cold-water immersion (3, 4). Both these

processes are key for stimulating muscle protein synthesis (which is important for repairing

muscle damage and the muscle growth response). Therefore, our question was: “how good

is cold-water immersion actually when looking at the muscle protein synthesis response

during recovery from exercise?”

In order to answer this question, we decided to conduct an acute as well as longer-term

experiment (5).

Acute (single day) muscle protein synthesis experiment

We recruited 12 healthy young male adults that were familiar with resistance-type exercise.

We asked them to come to our laboratory and let them perform a resistance-type exercise

session followed by one leg that was immersed (for 20 minutes) into cold water (8°C) and

the other leg into thermoneutral water (30°C). Immediately after water immersion we also

gave 20 grams of an intrinsically labeled (milk) protein shake. This “intrinsically labeled”

means that we had some amino acids within that protein shake “specifically labeled” so we

could see exactly where those amino acids would end up in the body and, particularly, in the muscles. Together with an infusion with tracers and taking blood and muscle samples for 5 hours after post-exercise water immersion, we could then exactly see what happened to the amino acids from the protein shake into our muscles and what happened to muscle protein synthesis.

What did we find?

When looking at what happened to the amino acids from the protein shake, we observed

that substantially less of these amino acids were being incorporated into the leg muscle that

was cooled (8°C) compared to the leg that recovered at the normal (30°C) temperature.

Cold water immersion with one leg in water of 8 degrees C and one leg in water of 30 degrees C

In addition, we also observed a clear impairment in post-exercise muscle protein synthesis in the cooled leg, compared to the thermoneutral leg (30°C) over the first 5 hours of post-exercise recovery. Therefore, this clearly shows a negative effect of post-exercise cold-water immersion on muscle protein synthesis during recovery from exercise.

As most people are obviously mostly interested in seeing whether their long-term gains

would be impacted (or not) by cooling as a recovery strategy, we also wanted to know

whether these acute findings would translate into longer-term findings.

Longer-term (2-week) muscle protein synthesis experiment

We also applied a 2-week experiment, where we asked the same individuals to come to our

lab during a 2-week period to perform 7 exercise sessions (on non-consecutive days) after

which we again performed the same cold water immersion procedure (20 min of one leg in

cold (8°C) and one leg in thermoneutral (30°C) water). Subsequently, we also provided a 20

g protein shake after every session. By providing our participants every day with an oral

tracer, we could determine muscle protein synthesis over the 2-week period.

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What did we find?

We observed similar findings as in our acute experiment, as we again found that muscle

protein synthesis was substantially lower (by 12%) over the full 2-week period in the leg that

was cooled after every exercise session compared to the thermoneutral leg. So, we showed

indeed that the acute (negative) effects of post-exercise cold-water immersion translate

into the longer-term.

Cold-water immersion and gains in muscle mass and strength

So far, we have been talking about muscle protein synthesis, which is (as I mentioned) an

important process for repairing muscle damage and the muscle growth response. However,

albeit being an important component, increases in muscle protein synthesis do not

necessarily reflect gains in muscle mass over a long-term period. Therefore, it is also

important to look at studies that actually measured gains in muscle mass (and strength)

over even more prolonged periods. In line with our muscle protein synthesis findings,

previous well conducted experiments have indeed shown that post-exercise cold water

immersion also attenuates skeletal muscle fiber hypertrophy (6), as well as gains in muscle

mass and strength (4).

Effects of cold water immersion (ice bath) on muscle protein synthesis after one sessions and after repeated session


Despite many athletes still performing cold-water immersion, based on well conducted

experiments, we can now conclude that if the goal is to increase gains in muscle mass and

strength you should avoid cold-water immersion as a strategy for post-exercise recovery.

Join the webinar (or recordings) through this link.

Join the webinar on cold and hot water immersion for recovery



  2. Thesis Cas Fuchs “Strategies for Post-Exercise Recovery”:

  3. Figueiredo VC, Roberts LA, Markworth JF, Barnett MP, Coombes JS, Raastad T, et al. Impact of resistance exercise on ribosome biogenesis is acutely regulated by post- exercise recovery strategies. Physiol Rep. 2016;4(2)

  4. Roberts LA, Raastad T, Markworth JF, Figueiredo VC, Egner IM, Shield A, et al. Post-exercise cold water immersion attenuates acute anabolic signalling and long-term adaptations in muscle to strength training. J Physiol. 2015;593(18):4285-301

  5. Fuchs CJ, Kouw IWK, Churchward-Venne TA, Smeets JSJ, Senden JM, Lichtenbelt W, et al. Postexercise cooling impairs muscle protein synthesis rates in recreational athletes. J Physiol. 2020;598(4):755-72

  6. Fyfe JJ, Broatch JR, Trewin AJ, Hanson ED, Argus CK, Garnham AP, et al. Cold water immersion attenuates anabolic signaling and skeletal muscle fiber hypertrophy, but not strength gain, following whole-body resistance training. J Appl Physiol (1985). 2019;127(5):1403-18

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